Antiproliferative activity and caspase enhancement properties of Annona muricata leaves extract against colorectal cancer cells
Item
Title
Antiproliferative activity and caspase enhancement properties of Annona muricata leaves extract against colorectal cancer cells
list of authors
1. Lili Indrawati
2. Purwantyastuti Ascobat
3.Budiman Bela
4. Murdani Abdullah
5. Ingrid S. Surono
6. Suwijiyo Pramono
2. Purwantyastuti Ascobat
3.Budiman Bela
4. Murdani Abdullah
5. Ingrid S. Surono
6. Suwijiyo Pramono
Abstract
Abstrak
Latar Belakang: Prevalensi kanker kolorektal meningkat di Asia termasuk di Indonesia. Annona muricata secara tradisional digunakan untuk menjaga kesehatan dan akhir-akhir ini digunakan untuk pasien kanker. Tujuan penelitian ini adalah untuk mengetahui efek antiproliferatif ekstrak daun A. muricata dan kemampuannya meningkatkan aktivitas caspase in vitro dan ex vivo.
Kata kunci: daun A. muricata, aktivitas sitotoksik in vitro dan ex vivo, sel kanker kolorektal,
Abstract
Background: The prevalence of colorectal cancer is rising in Asia including Indonesia. Annona muricata tea leaves, that is traditionally used for maintaining health, and lately being used by cancer patients. The objectives of this study is to investigate its effects in human colorectal cancer cell in vitro and ex vivo.
Methods: Thirty patients with colorectal cancer (CRC) were enrolled in a randomized double-blind placebo-controlled trial. They were equally divided into two groups: those treated with 300 mg A. muricata leaf extract and placebo daily for 8 weeks. Serum from supplemented CRC patients of both groups was compared for caspase 9 and caspase 8 enhancement activity. Antiproliferative effect of water extract of A. muricata leaves and its fractions were evaluated against colorectal cancer cell line (DLD-1 and COLO 205) compared with 5-Fluorouracil and placebo, the dose range were 62,5-2000 µg/mL. Method used was 3- (4, 5-dimethylthiazol- 2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. Data was analyzed by Mann-Whitney U test. The p-value was set at 0.05.
Conclusion: The study results suggest the cytotoxicity potential of A muricata leaves extract in in vitro and ex vivo studies.
Latar Belakang: Prevalensi kanker kolorektal meningkat di Asia termasuk di Indonesia. Annona muricata secara tradisional digunakan untuk menjaga kesehatan dan akhir-akhir ini digunakan untuk pasien kanker. Tujuan penelitian ini adalah untuk mengetahui efek antiproliferatif ekstrak daun A. muricata dan kemampuannya meningkatkan aktivitas caspase in vitro dan ex vivo.
Kata kunci: daun A. muricata, aktivitas sitotoksik in vitro dan ex vivo, sel kanker kolorektal,
Abstract
Background: The prevalence of colorectal cancer is rising in Asia including Indonesia. Annona muricata tea leaves, that is traditionally used for maintaining health, and lately being used by cancer patients. The objectives of this study is to investigate its effects in human colorectal cancer cell in vitro and ex vivo.
Methods: Thirty patients with colorectal cancer (CRC) were enrolled in a randomized double-blind placebo-controlled trial. They were equally divided into two groups: those treated with 300 mg A. muricata leaf extract and placebo daily for 8 weeks. Serum from supplemented CRC patients of both groups was compared for caspase 9 and caspase 8 enhancement activity. Antiproliferative effect of water extract of A. muricata leaves and its fractions were evaluated against colorectal cancer cell line (DLD-1 and COLO 205) compared with 5-Fluorouracil and placebo, the dose range were 62,5-2000 µg/mL. Method used was 3- (4, 5-dimethylthiazol- 2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. Data was analyzed by Mann-Whitney U test. The p-value was set at 0.05.
Conclusion: The study results suggest the cytotoxicity potential of A muricata leaves extract in in vitro and ex vivo studies.